Figure 1.

The role of geometry and plithotaxis in inducing motion-stress alignment. (a) Velocity angle (α) and stress orientation (β), calculated in relation to the direction defined by the direction of monolayer expansion (blue line). Scale bar = 100 μm. (b) Velocity and stress representation. The values σ_max and σ_min define the principal axis of the stress tensor. θ is the motion-stress alignment angle. (c) Distribution of motion-stress alignment angle (θ). (d) Distribution of velocity angle (α, left), stress orientation (β, right), where 0° represents alignment with the x axis (equivalent to direction of monolayer expansion). (e) Joint distribution of velocity angles and stress orientations accumulated for all monolayer locations over time. (f) Joint distribution of plithotaxis index and geometry index for all time points (n = 96) in all experiments (N = 4), estimated corresponding angle in Fig. S2. See Fig. S1 and Materials and Methods for a definition of the indices.