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. 2015 Dec 15;109(12):2562–2573. doi: 10.1016/j.bpj.2015.11.003

Figure 4.

Figure 4

Beating to nonbeating transition captured in single-cell droplet time series. (a) Plot of ATP consumed versus time elapsed during measurement for two different sperm (circles and triangles), both in the presence of 20 μM ATP. The time interval during which beating ceases is indicated in gray. Dashed line is linear fit to the data over the actively beating portion of the time series. α and β are the ATP consumption rates before and after beating stops, respectively, determined from linear fits of the data. For the two different sperm: β1/α1 = 0.84 and β2/α2 = 0.61. (b) Ratio of nonbeating to beating ATP consumption rate β/α as a function of [ATP] for all data exhibiting a beating to nonbeating transition (N = 12). (c) Histogram of β/α values (N = 12) for the beating to nonbeating transition data. The measured values range between 0.5 and 1.0 with a pronounced peak at ∼0.85. To see this figure in color, go online.