Figure 5.

Boxplot of ATP consumption rate measured for active sperm (N = 3), inactive sperm (N = 6), active WT Chlamydomonas axoneme (N = 10), inactive WT Chlamydomonas axoneme (N = 10), and dynein-deficient Chlamydomonas WS4 mutant (N = 10). All experiments were performed in reactivation buffer with [ATP] = 50 μM (see Materials and Methods). The thick line denotes the sample median of the distribution, and the circle is the sample mean. Lower and upper boundaries of the shaded box region denote the first and third quartile, respectively. Whisker boundaries denote minimum and maximum values for each distribution.