Figure 6.

IICR Activity Regulates the Expression Level and Clustering of Ca²⁺-Dependent PKC

(A) Protein expression levels of α, β2, and γ PKC subtypes and GABA_AR β3 subunit after 60-min exposure of neurons to DMSO or 2APB are shown.

(B) Quantification of the PKC/GABA_AR β3 subunit protein level ratio showing the PKCγ/GABA_AR β3 ratio significantly decreased after 2APB exposure for 60 min. Values (mean ± SEM) were normalized to the respective DMSO control condition (n = 6; ^∗∗∗p < 0.005, t-test).

(C) Co-staining of GABA_AR γ2 subunit and α, β2, or γ PKC subtypes after 60 min of DMSO or 2APB treatment. Arrowheads in upper panels indicate the dendrites enlarged in lower panels. Color codes in lower panels are as follows: green, GABA_AR; magenta, α, β2, or γ PKC; and white, GABA_AR and PKC colocalized clusters. Scale bars, 10 μm. Note that 2APB decreased the PKC β2 and γ, but not α, isoform immunoreactivities.

(D) PKC fluorescence intensity per pixel below GABA_AR punctae. Values (mean ± SEM) were normalized to the respective DMSO control condition (n = 30 cells for PKCα and PKCβ2; n = 40 for PKCγ; ^∗∗∗p < 0.005, t-test).