Fig. 3.

Neurosteroids increase ATP level and MMP in APP and P301L cells. ATP levels and MMP were measured after neurosteroid treatment for 24 h at a concentration of 100 nM in APP cells (a–b) and P301L cells (c–d), respectively. Values represent the mean ± SEM (n = 12–18 replicates of three independent experiments) and were normalized to 100 % of untreated Mock cells (a–b) or untreated wtTau cells (c–d). The values for untreated APP (a–b) and P301L cells (c–d) were also indicated by a dashed line. One-way ANOVA and post hoc Dunnett’s multiple comparison test versus untreated Mock or wtTau, *P < 0.05; **P < 0.01; ***P < 0.001. P progesterone, E2 estradiol, E1 estrone, T testosterone, 3α 3α-androstanediol