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. 2015 Sep 16;363:129–145. doi: 10.1007/s00441-015-2272-y

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — MBCD decreases the amount of lipid in the DRM fraction. HPLC chromatograms showing the separation of molecular PC and SM species and cholesterol of DRM fractions derived from MDCK cells incubated with 0 mM (top) and 10 mM MBCD (bottom). Note that the PC and SM species composition does not change after MBCD treatment and that cholesterol is specifically extracted from the DRM fraction. Both traces are plotted on the same scale and represent DRMs from an identical amount of cells. The left inset shows the decrease in lipids after MBCD treatment and the right inset shows the MBCD-mediated, dose-dependent depletion of cholesterol from the DRM fraction. Similar results with porcine sperm have been published previously (van Gestel et al. 2005a)