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. 2015 Oct 13;23(12):1843–1853. doi: 10.1038/mt.2015.166

Figure 5.

Figure 5

Cancer upregulated drug-resistant (CUDR) promotes highly upregulated in liver cancer (HULC) expression. (a) Methylation-specific polymerase chain reaction analysis on promoter region of HULC in hepatocyte-like cells with CUDR overexpression or control (indicated in upper). INPUT as internal control. Methyl DNA fragment is 180 bp. Unmethyl DNA fragment is 140 bp. (b) HULC promoter luciferase activity assay in hepatocyte-like cells with CUDR overexpresion or control. Data are means of values from three independent experiments, bar ± standard error of the mean. ** means P < 0.01. (c) Nuclear run-on analysis of HULC expression in induced hepatocyte-like cells with CUDR overexpression or knockdown (indicated in upper). β-actin as internal control. (d) Reverse-transcription polymerase chain reaction analysis of HULC in derived hepatocyte-like cells with CUDR overexpression or knockdown (indicated in upper). β-actin as internal control.