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. 2016 Jan 5;6:1479. doi: 10.3389/fmicb.2015.01479

FIGURE 2.

FIGURE 2

Multiple sequence alignment of: cyanide dihydratase from Bacillus pumilus C1 and Pseudomonas stutzeri (CynDpum, CynDstut) (Meyers et al., 1993; Jandhyala et al., 2003; Sewell et al., 2003), oxy-nitrilase from Synechocystis PCC6803 (3WUY) (Zhang et al., 2014), nitrilase from R. rhodochrous J1 (RRJ1) (Thuku et al., 2007), C-shaped β-alanine-synthase (βaS) from Drosophila melanogaster (2VHI) (Lundgren et al., 2008), and non spiral-forming crystallized nitrilase superfamily member 2PLQ (Nakai et al., 2000; Pace et al., 2000; Wang et al., 2001; Kumaran et al., 2003; Kimani et al., 2007). Sequence insertion regions in the spiral-forming nitrilases proposed to participate in the C-surface interaction leading to spiral formation are highlighted. Region 1 is highlighted in red and region 2 is highlighted in green. The putative catalytic residues are outlined. The sequence highlighted in blue also forms part of the interface and contributes a glutamate to the active site. The multiple sequence alignment was constructed with ClustalW2 (Larkin et al., 2007; Goujon et al., 2010). Alignment was edited and exported using Jalview software (Waterhouse et al., 2009).