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. 2016 Jan 5;6:1479. doi: 10.3389/fmicb.2015.01479

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Copyright © 2016 Park, Mulelu, Sewell and Benedik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gel filtration analysis on Superdex 200 10/300 GL of purified CynD protein in 100 mM MOPS pH 7.7. Elution monitored as absorbance at 220 nm. Vertical gray lines highlight prominent peaks. Continuous black line indicates wild-type CynD elution peak. Representative elution patterns of substitution mutants in CynD; (A) wild type 18-mer CynD, (B) intermediate 16-mer mutants (F69C shown) (see Table 2), (C) P. stutzeri CynD 14-mer-like F57C, (D) multiple elution peaks of R67C (also see Figure 6A), (E) sloping void peak of Q228C.