Table 1.
Primers used in the construction of the substitution mutations.
| C-surface regions site directed mutagenesis primers | |
|---|---|
| Pum Y54C F | GAA GCA TTT TTA CCT GGT TGC CCT TGG TTT GCT TTT ATT G |
| Pum Y54C R | CAA TAA AAG CAA ACC AAG GGC AAC CAG GTA AAA ATG CTT C |
| Pum P55C F | GCA TTT TTA CCT GGT TAT TGC TGG TTT GCT TTT ATT GG |
| Pum P55C R | CCA ATA AAA GCA AAC CAG CAA TAA CCA GGT AAA AAT GC |
| Pum W56C F | CCT GGT TAT CCT TGC TTT GCT TTT ATT GG |
| Pum W56C R | CCA ATA AAA GCA AAG CAA GGA TAA CCA GG |
| Pum F57C F | CTG GTT ATC CTT GGT GTG CTT TTA TTG GTC |
| Pum F57C R | GAC CAA TAA AAG CAC ACC AAG GAT AAC CAG |
| Pum A58C F | GGT TAT CCT TGG TTT TGT TTT ATT GGT CAT CC |
| Pum A58C R | GGA TGA CCA ATA AAA CAA AAC CAA GGA TAA CC |
| Pum F59C F | CCT TGG TTT GCT TGT ATT GGT CAT CC |
| Pum F59C R | GGA TGA CCA ATA CAA GCA AAC CAA GG |
| Pum I60C F | GTT ATC CTT GGT TTG CTT TTT GTG GTC ATC CAG AAT ATA CG |
| Pum I60C R | CGT ATA TTC TGG ATG ACC ACA AAA AGC AAA CCA AGG ATA AC |
| Pum G61C F | CCT TGG TTT GCT TTT ATT TGT CAT CCA GAA TAT ACG |
| Pum G61C R | CGT ATA TTC TGG ATG ACA AAT AAA AGC AAA CCA AGG |
| Pum H62C F | GTT TGC TTT TAT TGG TTG TCC AGA ATA TAC GAG |
| Pum H62C R | CTC GTA TAT TCT GGA CAA CCA ATA AAA GCA AAC |
| Pum P63C F | GTT TGC TTT TAT TGG TCA TTG CGA ATA TAC GAG AAA GTT C |
| Pum P63C R | GAA CTT TCT CGT ATA TTC GCA ATG ACC AAT AAA AGC AAA C |
| Pum E64C F | GCT TTT ATT GGT CAT CCA TGT TAT ACG AGA AAG TTC TAT C |
| Pum E64C R | GAT AGA ACT TTC TCG TAT AAC ATG GAT GAC CAA TAA AAG C |
| Pum Y65C F | GGT CAT CCA GAA TGT ACG AGA AAG TTC |
| Pum Y65C R | GAA CTT TCT CGT ACA TTC TGG ATG ACC |
| Pum T66C F | GGT CAT CCA GAA TAT TGC AGA AAG TTC TAT CAT G |
| Pum T66C R | CAT GAT AGA ACT TTC TGC AAT ATT CTG GAT GAC C |
| Pum R67C F | GTC ATC CAG AAT ATA CGT GCA AGT TCT ATC ATG |
| Pum R67C R | CAT GAT AGA ACT TGC ACG TAT ATT CTG GAT GAC |
| Pum K68C F | GTC ATC CAG AAT ATA CGA GAT GCT TCT ATC ATG A |
| Pum K68C R | TCA TGA TAG AAG CAT CTC GTA TAT TCT GGA TGA C |
| Pum F69C F | ATC CAG AAT ATA CGA GAA AGT GCT ATC ATG AAT TAT |
| Pum F69C R | ATA ATT CAT GAT AGC ACT TTC TCG TAT ATT CTG GAT |
| Pum Y70C F | CAG AAT ATA CGA GAA AGT TCT GCC ATG AAT TAT ATA AAA ATG C |
| Pum Y70C R | GCA TTT TTA TAT AAT TCA TGG CAG AAC TTT CTC GTA TAT TCT G |
| Pum H71C F | TAT ACG AGA AAG TTC TAT TGC GAA TTA TAT AAA AAT GCC G |
| Pum H71C R | CGG CAT TTT TAT ATA ATT CGC AAT AGA ACT TTC TCG TAT A |
| Pum E72C F | CGA GAA AGT TCT ATC ATT GCT TAT ATA AAA ATG CCG |
| Pum E72C R | CGG CAT TTT TAT ATA AGC AAT GAT AGA ACT TTC TCG |
| Pum E222C F | CGG AAG AAA TGA AAT GCA TGA TTT GTT TAA CG |
| Pum E222C R | CGT TAA ACA AAT CAT GCA TTT CAT TTC TTC CG |
| Pum M223C F | GAA ATG AAA GAG TGC ATT TGT TTA ACG CAG |
| Pum M223C R | CTG CGT TAA ACA AAT GCA CTC TTT CAT TTC |
| Pum I224C F | GAA ATG AAA GAG ATG TGC TGT TTA ACG CAG |
| Pum I224C R | CTG CGT TAA ACA GCA CAT CTC TTT CAT TTC |
| Pum C225A F | GAA AGA GAT GAT TGC TTT AAC GCA GGA G |
| Pum C225A R | CTC CTG CGT TAA AGC AAT CAT CTC TTT C |
| Pum L226C F | GAG ATG ATT TGT TGC ACG CAG GAG CAA AG |
| Pum L226C R | CTT TGC TCC TGC GTG CAA CAA ATC ATC TC |
| Pum K221C F | TAC GGA AGA AAT GTG CGA GAT GAT TTG |
| Pum K221C R | AAC AAA TCA TCT CGC ACA TTT CTT CCG |
| Pum T227C F | GAT GAT TTG TTT ATG CCA GGA GCA AAG AG |
| Pum T227C R | CTC TTT GCT CCT GGC ATA AAC AAA TCA TC |
| Pum Q228C F | GAT GAT TTG TTT AAC GTG TGA GCA AAG AGA TTA C |
| Pum Q228C R | GTA ATC TCT TTG CTC ACA CGT TAA ACA AAT CAT C |
| Pum E229C F | GAT TTG TTT AAC GCA GTG CCA AAG AGA TTA CTT TG |
| Pum E229C R | CAA AGT AAT CTC TTT GGC ACT GCG TTA AAC AAA TC |
| Pum Q230C F | GTT TAA CGC AGG AGT GCA GAG ATT ACT TTG |
| Pum Q230C R | CAA AGT AAT CTC TGC ACT CCT GCG TTA AAC |
| Pum R231C F | AAC GCA GGA GCA ATG CGA TTA CTT TGA AAC |
| Pum R231C R | GTT TCA AAG TAA TCG CAT TGC TCC TGC GTT |
| Pum D232C F | CGC AGG AGC AAA GAT GTT ACT TTG AAA C |
| Pum D232C R | GTT TCA AAG TAA CAT CTT TGC TCC TGC G |
| Pum Y233C F | CAG GAG CAA AGA GAT TGT TTT GAA ACA TTT AAG |
| Pum Y233C R | CTT AAA TGT TTC AAA ACA ATC TCT TTG CTC CTG |
| Pum F234C F | GCA AAG AGA TTA CTG CGA AAC ATT TAA GAG C |
| Pum F234C R | GCT CTT AAA TGT TTC GCA GTA ATC TCT TTG C |
| Pum E235C F | CAA AGA GAT TAC TTT TGC ACA TTT AAG AGC GG |
| Pum E235C R | CCG CTC TTA AAT GTG CAA AAG TAA TCT CTT TG |
Primers designed Primers designed using PrimerX (http://www.bioinformatics.org/primerx/).