Table 2.
Elution patterns of each mutant investigated by gel filtration is shown by X.
| Peak pattern/mutant | wt | P55C | W56C | F57C | A58C | F59C | I60C | G61C | H62C | P63C | E64C | Y65C | T66C | R67C | K68C | F69C | Y70C | H71C | E72C |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18-mer | X | X | X | X | X | X | X | X | X | X | |||||||||
| ∼16-mer | X | X | X | X | X | X | |||||||||||||
| 14-mer | X | X | X | ||||||||||||||||
| Dimer, hexamer, dimer | X | ||||||||||||||||||
| Extended or aggregated | |||||||||||||||||||
| Peak pattern/mutant | wt | E222C | M223C | I224C | C225A | L226C | T227C | Q228C | E229C | Q230C | R231C | D232C | Y233C | F234C | E235C | ||||
| 18-mer | X | X | X | X | X | X | X | X | X | X | X | X | |||||||
| ∼16-mer | X | X | |||||||||||||||||
| 14-mer | |||||||||||||||||||
| Multiple smaller | |||||||||||||||||||
| Extended or aggregated | X | ||||||||||||||||||
Representative chromatographs of each elution pattern shown in Figure 3.