Figure 1.

ANDRO induces proliferation in the dentate gyrus of adult mice. Two-month-old mice were injected i.p. with 2 mg kg⁻¹ ANDRO or vehicle as control 3 times a week for 4 weeks, received a single dose of 100 mg kg⁻¹ BrdU the last day of treatment, and were sacrificed 24 h after BrdU injection. (a) Images show representative immunostaining of BrdU. Scale bar: 50 μm. The graph shows total number of BrdU-positive (BrdU+) cells in the whole SGZ of control and ANDRO-treated mice. Bars represent mean ± S.E. (n = 7 mice). (b) Representative immunostaining of Ki67 and NeuN. Scale bar: 100 μm. (c, d) Total number of Ki67-positive (Ki67+) cells in the SGZ of control and ANDRO-treated 2-month-old (c) or 10-month-old (d) mice. Bars represent mean ± S.E. (n = 7 (c) and n = 5 (d) mice). ^∗ P < 0.05, ^∗∗ P < 0.01, Student's t-test.