Figure 4.

ANDRO increases the generation of newborn granule cells in the hippocampus of adult mice. (a) Schematic representation of the experimental procedure. Two-month-old mice were injected i.p. with 2 mg kg⁻¹ ANDRO or vehicle as control 3 times a week for 2 weeks and then received a daily i.p. injection of 100 mg kg⁻¹ BrdU for 3 consecutive days and continued with the treatments for 4 weeks. (b) Representative immunostaining of Ki67 and NeuN after 6 weeks of treatment. Scale bar: 100 μm. The graph represents quantification of the total number of Ki67-positive (Ki67+) cells in the SGZ. (c) Representative immunostaining of BrdU and NeuN. Insets show higher magnifications of double-positive cells. Scale bar: 50 μm (d) Quantification of the total number of double-positive (BrdU+NeuN+) cells in the GCL of control and ANDRO-treated mice. Bars represent mean ± S.E. (n = 4 mice). ^∗ P < 0.05, Student's t-test.