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. 2015 Dec;4(6):743–751. doi: 10.3978/j.issn.2218-6751.2015.12.10

Figure 1.

Figure 1

Immunohistochemical labeling for programmed death ligand-1 (PD-L1) performed with an anti-PD-L1 {clone EPR1161 [2]} & PD-1 [clone (NAT105)] from Abcam (Cambridge, MA, USA) with HIER using citrate buffer & LSAB method (Dako, Carpinteria, CA, USA). (A) Melanoma cells showing strong, uniform reaction in tumor cells (magnification, ×200); (B) PD-L1 negative in tumor cells, there is a positive reaction in immune cells in the tumor stroma and TILs (magnification, ×200). anti-PD-L1, anti-programmed death-1; HIER, heat-induced epitope retrieval; LSAB, labeled streptavidin biotin; TILs, tumor-infiltrating lymphocytes.