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. 2016 Jan 5;6:1495. doi: 10.3389/fmicb.2015.01495

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Copyright © 2016 Schmidt, Etalo, de Jager, Gerards, Zweers, de Boer and Garbeva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Setup of experiment and effect of pure volatiles on Collimonas pratensis Ter291 and Serratia plymuthica PRI-2C swarming motility (0.6% wt/vol agar) (A,B) and swimming motility (0.3% wt/vol agar) (C,D) on water agar supplied with artificial root exudates. Control: media. Five microliters of washed overnight cultures of C. pratensis Ter291 and S. plymuthica PRI-2C was spotted in the center of a soft agar partitioned Petri dish containing the pure volatiles and incubated for 3 days at 20°C. As an indicator of motility the average swimming and swarming diameter (cm) was measured. The error bars represent standard errors of the mean of three independent biological replicates. The asterisks indicate statistically significant (P < 0.05) differences relative to the control.