Differential detection of T. cruzi and T. rangeli infection of triatomine's intestinal contents by (A) amplification of the conserved regions of the minicircle of kDNA and (B) D7a divergent region of the large subunit ribosomal RNA gene. CC, T. cruzi control; CR, T. rangeli control; CN, negative control. Genotyping of T. rangeli was carried out by (C) the amplification of the conserved regions of the minicircle of kDNA to identify among the groups KP1(+) and KP1(−) and (D) the intergenic spacer of the splice leader (SL) gene to characterize the lineage. TC, T. cruzi control; LA, lineage A control of T. rangeli; LC, lineage C control of T. rangeli; CN, negative control. Color images available online at www.liebertpub.com/vbz