Figure 2. AhR is functionally expressed in Flt3L/GM-CSF-stimulated bone marrow cells and CD11c⁺CD103⁺ DCs. BM cells were induced to differentiate into CD11c⁺CD103⁺ DCs with Flt3L for the first 7 days and with Flt3L plus GM-CSF for the last 2 days. Then, the cells (FGBMCs) or CD11c⁺CD103⁺ sorted from FGBMCs were assayed for mRNA expression by PCR: AhR (a) and Cyp1A1 (c). The purity of the CD11c⁺CD103⁺ population was assessed post sorting (b). DIM (1 µM) was added to the culture 1 h before the addition of Flt3L/GM-CSF.