Figure 2. The inhibitory effect of GV1001 on ENO1-induced pro-inflammatory cytokine production in Con A-activated PBMCs. Activated PBMCs were pre-treated with GV1001 (100 µ M) for 1 h and then stimulated with anti-ENO1 mAb (1 µg) for 1 h. MOPC21 was used as an isotype control. Stimulated PBMCs were cultured in a 24-well plate (1×10⁶/ml) for another 48 h. The culture supernatant of the stimulated PBMCs was collected, and (A) TNF-α, (B) IL-1β, and (C) IL-6 levels were measured by ELISA. Data are presented as the means±SD. ^*p<0.05, ^**p<0.01, ^***p<0.001.