Figure 1. Resistin increases invasiveness of human breast cancer cells.

(A) MDA-MB-231 cells were seeded on a 6-well plate, and incubated until confluent monolayers were formed. A cell-free space was created by scraping through the monolayer. Migration was induced by treatment with 0, 10, 25, or 50 ng/ml resistin for 24 h. (B) MDA-MB-231 cells were stained, and randomly chosen fields were photographed at 40× magnification. The number of cells invading the lower surface of the monolayer was counted. Data are presented as mean ± SD from 3 independent experiments. (C) MCF-7 cells were seeded on a 6-well plate and incubated until a confluent monolayer was formed. A cell-free space was created by scraping through the monolayer. Migration was induced by treatment with 0, 10, 25, or 50 ng/ml resistin for 24 h. (D) MCF-7 cells were stained, and randomly chosen fields were photographed at 100× . The number of cells invading the lower surface was counted. The mean ± S.D. of 3 independent experiments is presented. *P < 0.05 vs. untreated control.