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. 2016 Jan 5;6:18360. doi: 10.1038/srep18360

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Copyright © 2016, Macmillan Publishers Limited

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SPAK knockout mice had reduced levels of total and p-NCC in kidney tissues. Acidosis increased the expression of total and p-NCC in SPAK^+/+ mice, but not in SPAK^−/− mice (6 mice/group, 3 separate gels). *p < 0.05 vs. SPAK^+/+ mice; ^#p < 0.05 vs. SPAK^+/+ mice with acidosis. The blots and gels were cropped and the full-length blots/gels are presented in Supplementary Fig. S4.