Figure 5. CREB and NF-κB are essential for the production of IL-8 by endothelial cells exposed to TpF1.

HUVECs were pre-incubated, or not, for 30 min with the CREB inhibitor KG-501 (a) or the NF-κB inhibitor QNZ (b), before the exposure to TpF1 or vehicle. At the indicated time points, culture supernatants were collected and evaluated for their IL-8 content by ELISA. Data are expressed as mean ± S.D. of three independent experiments. ***p < 0.001.