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. 2016 Jan 5;6:18061. doi: 10.1038/srep18061

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative fluorescent images of live 8 dpf zebrafish immersed in BODIPY C5 for 1 hour. Images revealed a remarkable presence of SCFA in probiotic treated larvae (B–D) that exhibited higher fluorescent signal in the intestine and gallbladder compared to the control one (A–C). Red asterisk: gallbladder; I: intestine. Scale bar: 500 μm. Probiotic treated larvae display significantly enhancement of fluorescent signal in the gallbladder (E) and in the intestine (F) with respect to the control group, highlighting an accumulation of short chain fatty acids (SCFA). Quantification of fluorescence is expressed in a.u. The data are reported as mean ± s.d from 3 individual experiments. The significance of differences between groups for these values was determined using Student’s t-test. Data are the mean ± s.d. Asterisks indicates significant differences (P < 0.05).