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. 2016 Jan 5;6:18706. doi: 10.1038/srep18706

Figure 1. Effect of different concentrations of morphine on cisplatin-inhibited proliferation of CNE-2 cells.

Figure 1

(A) Cells were treated with cisplatin (CDDP) 4 μg/ml for 24 h, 48 h, and 72 h. (B) Cells were treated without or with morphine (Mor) (0.1 μg/ml, 1 μg/ml, 10 μg/ml, 100 μg/ml or 1000 μg/ml) for 24 h, 48 h, and 72 h. (C) Cells were treated with cisplatin (CDDP) 4 μg/ml for 24 h in the presence of an increasing concentration of morphine (Mor). (D) Cells were treated with cisplatin (CDDP) 4 μg/ml for 24 h in the presence of an increasing concentration of morphine (Mor). (E) Cells were treated with cisplatin (CDDP) 4 μg/ml for 24 h in the presence of an increasing concentration of morphine (Mor). Cell viability was determined using the MTT assay as described in the Materials and Methods section. *P < 0.05, **P < 0.01. Error bars represent the mean ± SD of triplicates.