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. 2016 Jan 5;6:18706. doi: 10.1038/srep18706

Figure 2. Effect of the different concentrations of morphine on cisplatin-induced apoptosis in the CNE-2 cells.

Figure 2

Cells were treated with cisplatin (CDDP) 4 μg/ml for 72 h in the absence or presence of an increasing concentration of morphine (Mor). Thereafter, the cells were stained with Annexin V/PI and detected by fluorescence microscopy. (A) The apoptosis rate of the cells was determined as described in the Materials and Methods section. (B) The survival rate of the cells was determined as described in the Materials and Methods section. *P < 0.05, ***P < 0.001. Error bars represent the mean ± SD of triplicates.