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. Author manuscript; available in PMC: 2016 May 26.
Published in final edited form as: Biochemistry. 2015 May 12;54(20):3183–3196. doi: 10.1021/acs.biochem.5b00106

Table 1.

Cell-Based Assays Using E. coli DH10B pBCSK(−), Producing VIM-2, VIM-24 (R228L), and Other Variants

MIC (mg/L)
E. coli pBC SK(−) VIM-2 (228R) VIM-24 (R228L) R228K R228P R228M R228Q R228Y R228Xc
ampicillin   4 512 512 32 32 1024 1024 1024 64–512
cephalothin   8 256 256 16 16   256 1024   256 64–256
ceftriaxone   0.5     8     8   1   0.5     16     32     16 1–16
cefotaxime   0.06     8   16   1   1     16     32     16 2–32
aztreonam ≤0.125   ≤0.125   ≤0.125 ≤0.125 ≤0.125     ≤0.125     ≤0.125     ≤0.125 ≤0.125
ceftazidime   0.06   16   64   1   1   128   128     64 16–128
ceftazidime + Zna ≤1   32 128 ≤1   4   256   512   128 32–256
ceftazidime + EDTAb ≤1   16   32 ≤1 ≤1     64   128     64 8–64
cefepime   0.03     0.5     4   0.5   0.25       2     16       8 1–4
cefepime + Zna ≤0.25     0.5     4 ≤0.25   0.5       2     32       8 1–4
cefepime + EDTAb ≤0.25     0.5     4 ≤0.25 ≤0.25       4     16       8 1–2
imipenem   0.25     0.5     0.5   0.25   0.5       2       4       1 0.25–1
imipenem + Zna   0.25     1     1   0.25   0.25       4     >4       1 0.5–2
imipenem + EDTAb ≤0.125     0.5     0.5   0.25 ≤0.125       1       4       1 0.25–1
a

MHA supplemented with a final concentration of 250 μM Zn2+.

b

MHA supplemented with 5 μM EDTA.

c

Values shown in this column are the ranges of the MIC values obtained for the other variants. A complete list of the MICs is provided as Supporting Information.