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. Author manuscript; available in PMC: 2016 May 26.

Published in final edited form as: Biochemistry. 2015 May 12;54(20):3183–3196. doi: 10.1021/acs.biochem.5b00106

Table 2.

Steady-State Kinetic Data of VIM-2 and VIM-24^a

substrate	VIM-2			VIM-24
substrate	K_M (μM)	k_cat (s⁻¹)	k_cat/K_M (M⁻¹ s⁻¹)	K_M (μM)	k_cat (s⁻¹)	k_cat/K_M (M⁻¹ s⁻¹)
nitrocefin	13 ± 1	430 ± 20	3.3 × 10⁷	11 ± 1	171 ± 4	1.6 × 10⁷
ampicillin	23 ± 4	217 ± 9	9.9 × 10⁶	97 ± 6	217 ± 5	2.2 × 10⁶
cephalothin	17 ± 2	168 ± 8	9.8 × 10⁶	33 ± 5	150 ± 9	4.5 × 10⁶
ceftazidime	NM^c	NM^c	1 × 10⁴^b	NM^c	NM^c	6 × 10⁴^b
cefepime	NM^c	NM^c	3 × 10⁴^b	NM^c	NM^c	1 × 10⁵^b
imipenem	12 ± 1	45 ± 1	3.6 × 10⁶	5.0 ± 0.7	8.8 ± 0.3	1.7 × 10⁶

^a

Values reported are averages ± standard deviations from triplicate experiments.

^b

Calculated from the progress curves of reaction, as described in Experimental Procedures.

^c

Not measured.