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. Author manuscript; available in PMC: 2016 May 26.
Published in final edited form as: Biochemistry. 2015 May 12;54(20):3183–3196. doi: 10.1021/acs.biochem.5b00106

Table 3.

Inhibition of VIM-2 and VIM-24 by BTZsa

compound VIM-2
VIM-24
Ki (μM) Ki* (μM) k2 (s−1) k−2 (s−1) Ki (μM) Ki* (μM) k2 (s−1) k−2 (s−1)
L-CS319 3.7 ± 0.3 0.04 ± 0.01 (0.03 ± 1) × 10−3 (0.0003 ± 1) × 10−4 6.0 ± 0.7 0.12 ± 0.02 (0.06 ± 2) × 10−3 (0.001 ± 1) × 10−3
D-CS319 5.4 ± 0.4 0.18 ± 0.02 (0.01 ± 1) × 10−3 (0.0003 ± 1) × 10−4  11 ± 0.8 0.64 ± 0.01 (0.07 ± 4) × 10−3 (0.004 ± 1) × 10−3
L-VC26 3.8 ± 0.2 0.25 ± 0.01 (0.02 ± 1) × 10−3 (0.001 ± 1) × 10−3 4.9 ± 0.3 0.13 ± 0.02 (0.04 ± 2) × 10−3 (0.001 ± 1) × 10−3
D-VC26 14 ± 1   0.10 ± 0.03 (0.09 ± 4) × 10−3 (0.0006 ± 4) × 10−4 12 ± 3   0.08 ± 0.01 (0.01 ± 3) × 10−3 (0.00007 ± 1) × 10−5
a

Ki determined from the initial rate of hydrolysis (v0). Ki* and k2 were calculated from the analysis of the change in vs with inhibitor concentration, in the slow binding model.