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. 2016 Jan 5;6:18734. doi: 10.1038/srep18734

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Copyright © 2016, Macmillan Publishers Limited

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(A) Schematic illustration of hypoxia exposure and imaging. (B) Demonstration of live imaging of effects of hypoxic injury on motor neuron synaptic puncta visualized by FingR(PSD)-GFP in trunk of Tg(Hb9:Gal4); Tg(FingR(PSD95)-GFP) animals. Following hypoxia there is a decrease in the number of puncta labeled by FingR(PSD95)-GFP. Confocal images, rostral to left, scale bar 10 μm. (C) Confocal images and quantification in the dopaminergic neurons in the diencephalon of Tg(otpb.A:Gal4); Tg(FingR(PSD95)-GFP) animals. Hypoxia from 24–48 hpf decreased number of puncta but later hypoxia exposure did not significantly change number; (p = 0.002; one-way ANOVA; SEM shown). Confocal z-stacks, rostral to top, scale bar 10 μm.