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. 2016 Jan 5;6:18746. doi: 10.1038/srep18746

Figure 2. The effects of SPIONs cluster@PDA on cell viability, apoptosis and gene expression of ADSCs.

Figure 2

(A) Cell viability of ADSCs cultured with different concentrations of SPIONs cluster@PDA for 24 hrs and 48 hrs. Cell apoptosis was evaluated by both flow cytometry (B) and TUNEL assay (C) after incubation with 0.25 mM SPIONs cluster@PDA for 3 hrs, 6 hrs, 12 hrs and 24 hrs, respectively. Positive control: ADSCs treated with recombinant DNase I (300 U/ml in 50 mM Tris-HCl, pH 7.5, supplemented with 1 mg/ml BSA) for 10 min at 25 °C to induce cell apoptosis according to the manufacture’s protocol. Scale bar, 100 μm. (D) The relative gene expression of Nanog, OCT-4, SOX-2 and CXCR-4 of after incubation with 0.25 mM SPIONs cluster@PDA for 24 hrs.