Figure 4. Uptake of SPIONs cluster@PDA into ADSCs.
(A) Intracellular SPIONs cluster@PDA in ADSCs was detected by Prussian blue staining after the incubation with SPIONs cluster@PDA (a: control; b: for 3 hrs; c: for 6 hrs; d: for 12 hrs; e: for 24 hrs). Scale bar: 100 μm. (B) Intracellular iron content was quantificationally determined after incubated with 0.25 mM SPIONs cluster@PDA for 3 hrs, 6 hrs, 12 hrs and 24 hrs, respectively. (C) T2-weighted phantom images of ADSCs incubated with 0.25 mM SPIONs cluster@PDA for 3 hrs, 6 hrs, 12 hrs and 24 hrs, respectively.