Extended Data Figure 3. Comparison of wild-type lung neutrophils with tumour-induced, pre-metastatic lung neutrophils.

a, Representation of timing and dynamics of neutrophil and cancer cell infiltration into the lung of mice grafted with two mammary tumours by orthotopic injection of 10⁶ MMTV-PyMT tumour cells. b, Flow cytometric analysis for cell size (forward scatter (FSC)), granularity (side scatter (SSC)) and expression of surface markers CXCR2, CD31, MHC-I, MHC-II, ICAM1 and Fas (n is indicated) as well as mRNA expression analysis of Tnfa, arginase 1, Vegfa, Ccl2, Ccl3, iNOS (also known as Nos2) and Ccl5 by quantitative polymerase chain reaction (PCR) of CD11b⁺Ly6G⁺ wild-type (WT) or pre-metastatic (Pre-met.) lung neutrophils 3 weeks after primary tumour graft (n = 3 (pre-metastatic compared with one normal lung reference)). Statistical analysis by two-sided t-test (flow cytometry) and one-sample t-test (mRNA). Data are represented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.