Extended Data Figure 4. Immune cell frequencies and activation in the pre-metastatic lung of MMTV-PyMT tumour-bearing mice is not dependent on neutrophil presence (part 1).

a, Representation of timing and dynamics of neutrophil and cancer cell infiltration into the lung of mice grafted with two mammary tumours by orthotopic injection of 10⁶ MMTV-PyMT tumour cells. b–o, Flow cytometric quantification and representative analysis of the following immune cell types in wild-type (WT) or pre-metastatic (Pre-met.) lungs treated daily with either control IgG or anti-Ly6G (1A8) neutrophil-blocking antibody from tumour onset onwards (n = 4 per group if not otherwise indicated): b, f, total CD45⁺ immune cells (n = 12 per group); c, g, CD11b⁺Ly6G⁺ neutrophils (n = 8 per group); d, g, CD11b⁺SiglecF⁺ eosinophils; e, g, CD11b^lowF4/80^high alveolar macrophages and CD11b^highF4/80^low interstitial macrophages; h, j, CD45⁺CD11c⁺ dendritic cells; i, k, MHC-II⁺CD86⁺ activated dendritic cells; l, n, CD45⁺CD19⁺ B cells; and m, o, MHC-II⁺CD86⁺ activated B cells. Statistical analysis by two-sided t-test. Data are represented as mean ± s.e.m. NS, not significant, *P < 0.05, **P < 0.01, ***P < 0.001.