Extended Data Figure 5. Immune cell frequencies and activation in the pre-metastatic lung of MMTV-PyMT tumour-bearing mice is not dependent on neutrophil presence (part 2).

a–i, Flow cytometric quantification and representative analysis of the following immune cell types in wild-type (WT) or pre-metastatic (Pre-met.) lungs treated daily with either control IgG or anti-Ly6G (1A8) neutrophil-blocking antibody from tumour onset onwards (n = 4 per group if not otherwise indicated): a, c, CD45⁺CD49b⁺ NK cells; b, c, CD69⁺ activated NK cells; d, e, CD45⁺CD3⁺CD8⁺ cytotoxic T cells (n = 8 per group); f, g, CD44⁺ or CD69⁺ activated T cells; and h, i, the ratio of CD4⁺CD25⁺Foxp3⁺ regulatory T cells per activated T cell. Statistical analysis by two-sided t-test. Data are represented as mean ± s.e.m. NS, not significant.