Table 1.
Genetic diversity at microsatellite loci of sibling species E at six sampling sites
| Locus | Locations | |||||||
|---|---|---|---|---|---|---|---|---|
| Anu | Mon | Tha | Kan | Kat | Nik | Pooled population | ||
| N | 29 | 33 | 32 | 26 | 31 | 42 | 193 | |
| AcA59 | A | 8 | 11 | 11 | 10 | 9 | 9 | 14 |
| Rs | 7.896 | 10.686 | 10.432 | 10.000 | 8.829 | 7.901 | 9.830 | |
| F IS | 0.232* | 0.246* | 0.085* | 0.303* | −0.040* | 0.100* | 0.177* | |
| r | 0.1204 | 0.1311 | 0.0356 | 0.1662 | −0.0273 | 0.0458 | 0.1204 | |
| H e | 0.75983 | 0.88112 | 0.88641 | 0.82278 | 0.83818 | 0.76592 | 0.85555 | |
| H o | 0.58621 | 0.66667 | 0.81250 | 0.57692 | 0.87097 | 0.69048 | 0.70466 | |
| AcAVIB213 | A | 7 | 8 | 8 | 9 | 5 | 7 | 12 |
| Rs | 6.688 | 7.695 | 7.582 | 9.000 | 4.835 | 6.282 | 7.785 | |
| F IS | 0.059* | 0.202 | 0.072 | 0.332 | 0.048 | −0.026 | 0.136* | |
| r | 0.0213 | 0.1034 | 0.029 | 0.1865 | 0.0159 | −0.0187 | 0.0213 | |
| H e | 0.62250 | 0.68159 | 0.57192 | 0.74359 | 0.47382 | 0.58032 | 0.62312 | |
| H o | 0.58621 | 0.54545 | 0.53125 | 0.50000 | 0.45161 | 0.59524 | 0.53886 | |
| AcAIIB5 | A | 4 | 4 | 5 | 5 | 2 | 5 | 6 |
| Rs | 3.999 | 3.958 | 5.000 | 5.000 | 2.000 | 4.999 | 4.963 | |
| F IS | −0.387* | 0.660* | 0.326* | 0.201* | −0.176 | 0.387* | 0.319* | |
| r | −0.1672 | 0.4824 | 0.1847 | 0.1001 | −0.0877 | 0.2318 | −0.1672 | |
| H e | 0.65094 | 0.61678 | 0.69196 | 0.76697 | 0.27499 | 0.77281 | 0.71499 | |
| H o | 0.89655 | 0.21212 | 0.46875 | 0.61538 | 0.32258 | 0.47619 | 0.48705 | |
| AcAVb93 | A | 5 | 5 | 6 | 7 | 4 | 6 | 9 |
| Rs | 4.887 | 4.998 | 5.962 | 7.000 | 3.839 | 5.853 | 6.522 | |
| F IS | 0.345* | −0.285 | 0.120* | 0.096* | −0.426 | −0.008* | 0.047* | |
| r | 0.1972 | −0.1302 | 0.0548 | 0.04 | −0.1801 | −0.0101 | 0.1972 | |
| H e | 0.62795 | 0.56830 | 0.67312 | 0.76471 | 0.56954 | 0.73207 | 0.70161 | |
| H o | 0.41379 | 0.72727 | 0.59375 | 0.69231 | 0.80645 | 0.73810 | 0.66839 | |
| AcA36 | A | 6 | 4 | 5 | 5 | 2 | 6 | 8 |
| Rs | 5.990 | 4.000 | 4.807 | 5.000 | 2.000 | 5.217 | 5.664 | |
| F IS | 0.077 | 0.149 | −0.369 | 0.086 | −0.429 | −0.071 | −0.038 | |
| r | 0.0306 | 0.0718 | −0.1606 | 0.0342 | −0.181 | −0.0398 | 0.0306 | |
| H e | 0.67151 | 0.67506 | 0.64286 | 0.67195 | 0.43205 | 0.46730 | 0.60402 | |
| H o | 0.62069 | 0.57576 | 0.87500 | 0.61538 | 0.61290 | 0.50000 | 0.62694 | |
| All Loci | A | 6 | 6.4 | 7 | 7.2 | 4.4 | 6.6 | 9.8 |
| Rs | 5.892 | 6.267 | 6.756 | 7.2 | 4.299 | 6.24 | 6.953 | |
| F IS | 0.070 | 0.206 | 0.054 | 0.207 | −0.188 | 0.097 | 0.135 | |
| r | 0.0345 | 0.1002 | 0.0444 | 0.0825 | 0.0666 | 0.0233 | 0.0345 | |
| H e avg | 0.66655 | 0.68457 | 0.69325 | 0.75400 | 0.51772 | 0.66368 | 0.69986 | |
| H o avg | 0.62069 | 0.54545 | 0.65625 | 0.60000 | 0.61290 | 0.60000 | 0.60519 | |
N- number of samples, A = number of alleles, F IS – inbreeding coefficient, Rs – allele richness, r- null allele frequency, H e– expected heterozygosity, H o– observed heterozygosity, All loci, all samples – mean values over loci and populations. Probability test against HWE * p < 0.006 after Bonferroni correction for the pooled population and p < 0.001 for the sampling sites. Anu-Anuradhapura, Mon-Monaragala, Tha-Thanamalwila, Kan-Kandy, Kat-Kataragama, Nik-Nikaweratiya