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Primer sequences of the three single point mutations on AAV-DJ capsid
| Residue | Sequence (5’ to 3’) | Nucleotide change |
|---|---|---|
| K137R | Wild-type primer sequence: TGAGGAAGCGGCTAAGACG | AAG → AGG |
| Mutant primer sequence: TGAGGAAGCGGCTAGGACG | ||
| T251A | Wild-type primer sequence: CCCTGCCCACCTACAACAAC | ACC → GCC |
| Mutant primer sequence: CCCTGCCCGCCTACAACAAC | ||
| S503A | Wild-type primer sequence: GTGAATACTCGTGGACTGGAG | TCG → GCG |
| Mutant primer sequence: GTGAATACGCGTGGACTGGAG |
