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. 2016 Jan 4;13:1. doi: 10.1186/s12985-015-0456-4

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© León-Juárez et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Association of the NS2B protein with bacterial membranes. a Western blot analysis of NS2B protein expression in E. coli using an anti-V5 antibody. Lane 1: NS2B clone, uninduced. Lane 2: bacteria transformed with the parental vector. Lane 3: NS2B clone, induced with IPTG. Lane 4: Inclusion bodies of bacteria transformed with NS2B. Lane 5: purified NS2B protein. Lane 6: purified bacterial membranes. b and c western blot analysis of bacterial membrane fractions using a mouse polyclonal antibodies specific to-OmpCF (resident bacterial membrane protein) and α-V5. Lanes 1 and 2: purified protein NS2B. Lane 3: bacterial membrane fraction