Fig. 4.

NS2B protein expression arrests E. coli growth and affects the permeability of bacterial membranes. a Grow courve of E.coli BL21 DE3 transformed with pProEX/NS2B (toxicity control). Growth curves of engineered E. coli BL21pLys transformed with NS2B, pProEX, and PTB constructs in the absence b or presence c of IPTG. The cellular density of bacterial cultures was determined every 30 min after induction by measuring optical densities at 660 nm