Fig. 5.

E. coli BL21 pLysS transformed with NS2B a and PTB b expression plasmids. Both transformant groups were induced with IPTG in the absence (−) or presence (+) of HygB. Aliquots of these cultures were subjected to metabolic labeling with [³⁵S] Met-Cys at the indicated time points. The samples were resolved by SDS-PAGE and analyzed by autoradiography