Table 3.

SNVs in the pSOL1 megaplasmid of C. acetobutylicum ATCC 824 COSMIC strain and re-acquired ATCC 824 strain

NC_001988	Type	Ref	Allele	Feature	Change	AA Change	Function/comment
ATCC 824 isolate re-acquired from the ATCC in November 2011
18758	SNV	G	T	CA_P0017	11C > A	Ala4Glu	GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731
54266	SNV	A	G	CA_P0053	317T > C	Leu106Pro	Xylanase XYNB, now identical to SMB_P052 of DSM 1731
56219	SNV	A	G	CA_P0055	506A > G	Asn169Ser	Hypothetical protein, now identical to SMB_P054 of DSM 1731
60555	SNV	G	T	CA_P0059	754C > A	His252Asn	Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731
191997	Del	ACTC	–	Deletion	–	–	Same deletion as in pSMBa of DSM1731
ATCC 824 COSMIC strain
18758	SNV	G	T	CA_P0017	11C > A	Ala4Glu	GrkB germination protein, 4Glu also in SMB_P016 of DSM 1731
54266	SNV	A	G	CA_P0053	317T > C	Leu106Pro	Xylanase XYNB, now identical to SMB_P052 of DSM 1731
56219	SNV	A	G	CA_P0055	506A > G	Asn169Ser	Hypothetical protein, now identical to SMB_P054 of DSM 1731
60555	SNV	G	T	CA_P0059	754C > A	His252Asn	Alcohol dehydrogenase, now identical to SMB_P058 of DSM 1731
69715	Del	69715…74631		CA_P0066-71	del4916 bp	–	Unique to pSOL1, affects ptna, manY,ptnd, CA_P0069, CA_P0070, CA_p0071
191997	Del	ACTC	–	Deletion	–	–	Same deletion as in pSMBa of DSM1731
DSM 1731 (map positions are to NC_015686)
18524	SNV	C	A	SMB_P016	245G > T	Gly82Val	GrkB, germination protein, Gly at position 82 instead of Val in CA_P0017
20626	SNV	G	A	SMB_P019	220C > T	Ala407Val	GrkA, germination receptor, Ala at position 407 instead of Val in CA_P0020