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. 2015 Oct 20;6(6):336–340. doi: 10.1016/j.phrp.2015.10.003

Figure 1.

Figure 1

(A) Digestion of pTY-α-amylase with NheI and XhoI restriction enzymes. Lane 1: pTYB2 digested with NheI and XhoI; lane2: pTY-α-amylase digested with NheI and XhoI; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.