Table 1.
Concentration changes of analytes induced by repeated freezing and thawing of plasma samples.
| Analyte (pg/mL) |
Freeze–thaw cycles |
p | |||
|---|---|---|---|---|---|
| 2 cycles (baseline) | 3 cycles∗ | 4 cycles∗ | 5 cycles∗ | ||
| IFN-γ | 6.14 ± 8.80 | 6.07 ± 7.74 | 5.49 ± 5.17 | 5.45 ± 6.05 | 0.205 |
| (–1.2) | (–10.6) | (–11.3) | |||
| IL-8 | 96.35 ± 164.46 | 96.82 ± 164.79 | 93.96 ± 154.99 | 94.58 ± 159.41 | 0.548 |
| (+0.5) | (–2.5) | (–1.8) | |||
| IL-15 | 1.70 ± 1.05 | 1.72 ± 1.00 | 1.69 ± 1.11 | 1.67 ± 1.26 | 0.963 |
| (+1.0) | (–0.7) | (–1.7) | |||
| IL-17A | 1.24 ± 1.59 | 1.20 ± 1.54 | 1.13 ± 1.36 | 1.084 ± 1.21 | 0.495 |
| (–3.7) | (–9.4) | (–13.1) | |||
| MMP7 | 2.34 ± 1.91 | 2.57 ± 2.11† | 2.64 ± 2.18† | 2.71 ± 2.03† | <0.001 |
| (+9.9) | (+12.5) | (+15.6) | |||
| TNF-α | 48.55 ± 49.42 | 47.48 ± 47.41 | 47.56 ± 47.69 | 47.02 ± 47.56† | 0.072 |
| (–2.2) | (–2.0) | (–3.2) | |||
| VEGF | 134.39 ± 80.64† | 144.40 ± 90.81† | 148.67 ± 97.15† | 154.69 ± 104.38† | 0.124 |
| (+7.4) | (+10.6) | (+15.1) | |||
| VEGFR2 | 7,292.03 ± 878.64 | 7,259.46 ± 833.46 | 7,266.65 ± 866.95 | 7,278.96 ± 850.16 | 0.903 |
| (–0.4) | (–0.3) | (–0.2) | |||
IFN = interferon; IL = interleukin; MMP = matrix metalloproteinase; TNF = tumor necrosis factor; VEGF = vascular endothelial growth factor.
∗
Values within“( )” show the percentage change with a “+” for an increase and a “–” for a decrease compared with baseline.
†
Indicates p < 0.05 calculated using paired 2-tailed t test. The p value was measured using repeated-measures analysis of variance.