Table 1. In-life observations.
| Parameter | Method | Frequency | Number of Mice | Remarks |
|---|---|---|---|---|
| Body weight | Gravimetry | Twice weekly | All | Individually |
| In-life observations | Non-systematic observation | Daily | All | — |
| Group observations | Daily | All* | After exposure | |
| Individual observations according to checklist | Daily | One cage per exposure group | Immediately after removal from exposure chambers | |
| Mortality | Observation | Daily | All mice | — |
*With the exception of those mice selected for carboxyhemoglobin measurement or urine collection on the day of determination.