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. 2015 Dec 31;21:4122–4129. doi: 10.12659/MSM.896661

Figure 1.

Figure 1

MiR-129-5p sensitized breast cancer cells to irradiation, while irradiation induced-autophagy weakened radiosensitivity. (A) QRT-PCR analysis of the relative miR-129-5p expression in 4 breast cancer cell lines (MCF-7, MDA-MB-231, BT549, and BT474) and 1 non-tumorigenic breast epithelial cell line (MCF-10A). (B) QRT-PCR analysis of relative miR-129-5p expression in MDA-MB-231 cells transfected with miR-129-5p mimic or MCF-7 cells transfected with anti-miR-129-5p. (C, D) The survival fraction of MCF-7 cells (with or without miR-129-5p knockdown) (C) and MDA-MB-231 cells (with or without miR-129-5p overexpression) (D) after exposure to indicated doses. Colonies containing >50 cells were counted via microscopic inspection. (E) MCF-7 and MDA-MB-231 cells were exposed to 6-Gy irradiations. Autophagosome formation over time was detected via Western blot using antibodies against LC3 and p62. (F) Western blot analysis of autophagy in MCF-7 and MDA-MB-231 cells with or without treatment with 3-MA (5 mM) after irradiation (6-Gy) for 24 h. (G, H) The survival fraction of MCF-7 and MDA-MB-231 cells with 6-Gy exposure alone or with combined irradiation and 3-MA (5-mM) treatment. * p<0.05, ** p<0.01, *** p<0.001.