Fig. 5.

DNA synthesis and oriC occupancy of MtrA and DnaA in dnaAcos/MtrA_Y102C. The inducer ‘atc’ was added at the time of initiation of synchronous replication (0 h) to overproduce MtrA_Y102C. (A). DNA synthesis determined as described under Fig. 3A. (B). ChIP-PCR showing the MtrA occupancy of oriC performed as described for Fig. 3B. (C) ChIP-PCR showing the DnaA occupancy of oriC as described for Fig. 3C.