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ChIP-PCR studies with M. smegmatis lysates. (A) M. smegmatis producing MtrAY102C were processed for ChIP studies with MtrA antibodies essentially as described for Fig. 3C. Following the reversal of cross-links, PCR was performed for MtrA-targets oriC and PfbpB and the non-target PftsZ. (B) ChIP-PCR with M. smegmatis lysates carrying the M. smegmatis oriC (pMQ131) (Qin et al., 1997) and M. tuberculosis oriC (pMQ219) (Qin et al., 1999) in replicating plasmids.
