Table 1.

PRP system protocols summary.

System	Centrifugation or filtration	Volume of blood required	Anticoagulant	Volume of anticoagulant	Size of syringe used	PRP product volume
1	Centrifugation	50 mL	ACD-A	10 mL	60 mL	10 mL
2	Centrifugation	16 mL	ACD-A	1.5 mL	20 mL	4–7 mL
3	Centrifugation	50 mL	ACD-A	10 mL	60 mL	4 mL
4	Centrifugation	9 mL	Sodium citrate	1 mL	n/a	4–5 mL
5	Filtration	55 mL	ACD-A	5 mL	60 mL	6–8 mL

The following five commercial systems were prospectively analyzed and labeled as follows: System 1, System 2, System 3, System 4, and System 5. Blood was then obtained from each dog according to the manufacturer’s protocol and specifications for each PRP system. This table summarizes the main characteristics of each system’s protocol.