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. 2015 Dec 31;9(12):e0004318. doi: 10.1371/journal.pntd.0004318

Fig 2. S. mansoni gDNA detection on chip with LAMP.

Fig 2

(A) Images of fluorescence emission at t = 20, 48, and 58 min from three reaction chambers during on-chip LAMP amplification of 50 fg (left), 5 fg (middle), and 0 fg (no target control, right) S. mansoni gDNA. (B) Real time monitoring of Sm1-7 LAMP reactions with 50, 5, and 0.5 fg S. mansoni gDNA spiked in 20 μL plasma. The S. mansoni gDNA was captured on the silicon membrane and used as template. Matlab code was used to extract the averaged fluorescence intensity signals for each reactor. (C) The threshold time in B is depicted as a function of the amount of S. mansoni gDNA in 20 μL plasma, ± S.D. (n = 3). (D) Test of a sample with a very low concentration of 0.005 fg/μL S. mansoni gDNA at sample volumes of 200, 100, and 20 μL.