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. 2015 Dec 15;109(12):2592–2601. doi: 10.1016/j.bpj.2015.11.002

Figure 7.

Figure 7

Measured and predicted effects of increasing the Lp of N2B-Us on passive force. The predicted force-SL relation of a single titin molecule was calculated using a serially linked WLC model with an N2B-Us Lp of 0.3 nm (unphosphorylated state) or 0.6 nm (phosphorylated state). (For other parameters and additional details, see Materials and Methods.) The predicted force is reduced in the phosphorylated state. The percent force reduction is shown as a function of SL in the inset. The measured force reduction at SL 2.2 μm after CaMKIIδ and ERK2 phosphorylation (data from Fig. 6D) is also shown. The magnitude of the measured force reduction after CaMKIIδ phosphorylation is similar to the predicted value, but the measured ERK2 phosphorylation is considerably less than predicted.