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. Author manuscript; available in PMC: 2016 Jan 5.
Published in final edited form as: Mol Microbiol. 2015 Feb 18;96(2):325–348. doi: 10.1111/mmi.12939

Fig. 2.

Fig. 2

Effect of Rap60-Phr60 on sporulation and transcription of early sporulation genes.

A. Sporulation frequencies were measured for each strain and normalized to wild type strain JH642. Strains over-expressing Rap60 (KB26), Phr60 (KB28), Rap60-Phr60 (KB27) and empty vector (KB19) were treated with 0.1 mM IPTG. Strains containing pTA1060 (KG1780), pTA1060-Δphr60 (KG1782) and pTA1060-Δrap60-Δphr60 (KG1781) were grown in the presence of erythromycin to select for plasmid maintenance. Sporulation frequencies ranged from 20% to 40% for strain JH642. Experiments were performed in triplicate and the standard error is shown. Asterisks represent P-values < 0.01.

B-D. Cultures containing promoter fusions to lacZ were grown in DSM. Aliquots were removed at the specified time, and β-galactosidase activity was determined. Experiments were repeated in triplicate with similar results. A single representative experiment is shown.

B. Over-expression of Rap protein and Phr peptide from Pspank in strains containing PspoIIA-lacZ: KB45 empty vector (filled circle); KB63 RapC (open circle); KB48 Phr60 (filled triangle); KB47 Rap60-Phr60 (filled diamond); KB46 Rap60 (open square); and KB138 RapB (X).

C. Stains containing PspoIIA-lacZ and derivatives of plasmid pTA1060: KG1766 pTA1060 (filled circles); KG1772 pTA1060-Δphr60 (filled triangles); and KG1771 pTA1060-Δrap60-Δphr60 (filled diamond).

D. Strains containing Pskf-lacZ and derivatives of plasmid pTA1060: KG1911 pTA1060 (filled circles) and KG1912 pTA1060-Δphr60 (filled triangles).