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. Author manuscript; available in PMC: 2016 Jan 5.
Published in final edited form as: Mol Microbiol. 2015 Feb 18;96(2):325–348. doi: 10.1111/mmi.12939

Fig. 3.

Fig. 3

Effect of Rap60-Phr60 on colony morphology, pellicle formation and transcription of biofilm genes.

A. Colony morphology was determined by inoculating 10 µl of an overnight culture onto MSgg solid medium and incubating plates at 24°C for 3 days. Pellicle formation was determined by inoculating 10 µl of an overnight culture into 3 ml of liquid MSgg medium in 6-well microtiter plates, followed by incubation at 24°C for 3 days.

B-D. Cultures containing promoter fusions to lacZ and derivatives of pTA1060 were grown in MSgg medium. Aliquots were removed at the specified time and β-galactosidase activity was determined. Experiments were repeated in triplicate with similar results. A single representative experiment is shown.

B. Strains containing PespA-lacZ: KG1928 pTA1060 (filled circles) and KG1929 pTA1060-Δphr60 (filled triangles).

C. Strains containing PtapA-lacZ: KG1930 pTA1060 (filled circles) and KG1931 pTA1060-Δphr60 (filled triangles).

D. Strains containing PabrB-lacZ: KG1903 pTA1060 (filled circles) and KG1905 pTA1060-Δphr60 (filled triangles).